Studies on the association between beta 2-glycoprotein I and hepatotropism of hepatitis B virus / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To further study the binding character of hepatitis B surface antigen (HBsAg) and beta 2-glycoprotein I (beta2GP I) and to explore whether beta2GP I plays an important role in the hepatotropism of hepatitis B virus.</p><p><b>METHODS</b>Using Western blot technique, we observed the binding character of the HBsAg with reduced and non-reduced beta2GP I.</p><p><b>RESULTS</b>rHBsAgs with reduced and non-reduced beta2GP I showed identical binding activity.</p><p><b>CONCLUSIONS</b>The binding activity of HBsAg is dependent on tandem residues, but not on conformational structures of beta2GP I. There is a specific binding between HBV and beta2GP I, which may play an important role in HBV infection and is one of the reasons of hepatotropism of HBV.</p>

Preparation of anti-gastric cancer monoclonal antibody with daunorubicin methotrexate conjugate and its cytotoxiciy to gastric cancer cells / 中国免疫学杂志

It is first time to use dextren T-40 oxidative method to conjugate anti-gastric cancer mono-colonal antibody(McAb)with anti-tumor medicines of daunorubicin(DNR)and methotrexate(MTX)together.Cytotoxicity of conjugates was measured by MTT method and ~3H-TdR incor-poration method respectively.Both sensitivity is similar.The results have showed that this conju-gate exhibited selective cytotoxicity on human gastric cancer cells in vitro.